Product Description
Rat NE(Noradrenaline/Norepinephrine) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: NA; NAd; Norepinephrine; Norepi
Species: Rat
Assay Type: Sandwich
Sensitivity: 8 pg/mL
Standard: 400 pg/mL
Detection Range: 6.25-400 pg/mL
Sample Type: serum, plasma, cell lysates, cell culture supernates and other biological fluids
Assay Length: 2h
Research AreaResearch Area: Signal transduction;Infection immunity;Neuro science;Hormone metabolism;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Noradrenaline/Norepinephrine(NE). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Noradrenaline/Norepinephrine(NE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Noradrenaline/Norepinephrine(NE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Noradrenaline/Norepinephrine(NE) in the samples is then determined by comparing the OD of the samples to the standard curve.
