Product Description
Rat KLK1(Kallikrein 1) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: KLKR; Klk6; HK1; Kallikrein 1,Renal/Pancreas/Salivary; Kidney/pancreas/salivary gland kallikrein; Tissue kallikrein
Species: Rat
Assay Type: Competitive Inhibition
Sensitivity: 48.2 pg/mL
Standard: 10000 pg/mL
Detection Range: 156.25-10000 pg/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids
Assay Length: 2h
Research AreaResearch Area: Enzyme & Kinase;Tumor immunity;Hepatology;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Kallikrein 1(KLK1) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Kallikrein 1(KLK1). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Kallikrein 1(KLK1) in the samples is then determined by comparing the OD of the samples to the standard curve.
