Product Description
Rat Kim1(Kidney Injury Molecule 1) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: HAVCR1; TIM1; TIMD1; HAVCR; Hepatitis A Virus Cellular Receptor 1; T Cell Immunoglobulin And Mucin Domain-Containing Protein 1; T-cell immunoglobulin mucin receptor 1
Species: Rat
Assay Type: Sandwich
Sensitivity: 23.3 pg/mL
Standard: 4000 pg/mL
Detection Range: 62.5-4000 pg/mL
Sample Type: tissue homogenates, urine, cell culture supernates and other biological fluids
Assay Length: 4.5h
Research AreaResearch Area: Tumor immunity;Infection immunity;Kidney biomarker;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kidney Injury Molecule 1(Kim1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Kidney Injury Molecule 1(Kim1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Kidney Injury Molecule 1(Kim1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Kidney Injury Molecule 1(Kim1) in the samples is then determined by comparing the OD of the samples to the standard curve.
