Product Description
Rat IL7R(Interleukin 7 Receptor) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: CDW127; CD127; IL-7R-Alpha
Species: Rat
Assay Type: Sandwich
Sensitivity: 0.116 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Length: 4.5h
Research AreaResearch Area: Signal transduction;CD & Adhesion molecule;Infection immunity;Immunodeficiency;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 7 Receptor(IL7R). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 7 Receptor(IL7R). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 7 Receptor(IL7R), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 7 Receptor(IL7R) in the samples is then determined by comparing the OD of the samples to the standard curve.
