Product Description
Rat ICAM1(Intercellular Adhesion Molecule 1) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: CD54; BB2; P3.58; Human Rhinovirus Receptor; Major group rhinovirus receptor
Species: Rat
Assay Type: Sandwich
Sensitivity: 0.125 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 4.5h
Research AreaResearch Area: CD & Adhesion molecule;Tumor immunity;Infection immunity;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Intercellular Adhesion Molecule 1(ICAM1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Intercellular Adhesion Molecule 1(ICAM1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Intercellular Adhesion Molecule 1(ICAM1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Intercellular Adhesion Molecule 1(ICAM1) in the samples is then determined by comparing the OD of the samples to the standard curve.
