Product Description
Rat HSP90α(Heat Shock Protein HSP 90-alpha ) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: FLJ31884, HSP86, HSP89A, HSP90A, HSP90N, HSPC1, HSPCA, HSPCAL1, HSPCAL4, HSPN, Hsp89, Hsp90, LAP2, heat shock 90kD protein 1, alpha|heat shock 90kD protein 1, alpha-like 4|Heat shock protein HSP 90-alpha
Species: Rat
Assay Type: Sandwich
Sensitivity: 0.096ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: serum, plasma, tissue homogenates
Assay Length: 4.5h
Research AreaResearch Area: Neuro science,Stress response
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock Protein 90α（HSP90α）. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock Protein 90α（HSP90α）. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock Protein 90α（HSP90α）, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heat Shock Protein 90α（HSP90α） in the samples is then determined by comparing the OD of the samples to the standard curve.
