Product Description
Rat HRH4(Histamine Receptor H4) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: GPCR105; GPRv53; H4R; HH4R; H4; AXOR35; BG26; G-protein coupled receptor 105
Species: Rat
Assay Type: Sandwich
Sensitivity: 0.055 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids.
Assay Length: 4.5h
Research AreaResearch Area: CD & Adhesion molecule;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Histamine Receptor H4(HRH4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Histamine Receptor H4(HRH4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Histamine Receptor H4(HRH4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Histamine Receptor H4(HRH4) in the samples is then determined by comparing the OD of the samples to the standard curve.
