Product Description
Rat HPD(4-Hydroxyphenylpyruvate Dioxygenase) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: PPD; 4-HPPD; 4HPPD; GLOD3; Glyoxalase Domain Containing 3; 4-hydroxyphenylpyruvic acid oxidase
Species: Rat
Assay Type: Sandwich
Sensitivity: 1.35 ng/mL
Standard: 200 ng/mL
Detection Range: 3.13-200 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids.
Assay Length: 4.5h
Research AreaResearch Area: Enzyme & Kinase;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 4-Hydroxyphenylpyruvate Dioxygenase(HPD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 4-Hydroxyphenylpyruvate Dioxygenase(HPD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 4-Hydroxyphenylpyruvate Dioxygenase(HPD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 4-Hydroxyphenylpyruvate Dioxygenase(HPD) in the samples is then determined by comparing the OD of the samples to the standard curve.
