Product Description
Rat GP73(Golgi Protein 73) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: GOLM1; GOLPH2; C9orf155; Golgi Membrane Protein 1; Golgi Phosphoprotein 2
Species: Rat
Assay Type: Sandwich
Sensitivity: 0.31 ng/mL
Standard: 50 ng/mL
Detection Range: 0.79-50 ng/mL
Sample Type: serum, plasma, tissue homogenates and other biological fluids
Assay Length: 4.5h
Research AreaResearch Area: Tumor immunity;Infection immunity;Reproductive science;Hepatology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Golgi Protein 73(GP73). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Golgi Protein 73(GP73). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Golgi Protein 73(GP73), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Golgi Protein 73(GP73) in the samples is then determined by comparing the OD of the samples to the standard curve.
