Product Description
Rat EMMPRIN(Extracellular Matrix Metalloproteinase Inducer) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: CD147; BSG; 5F7; M6; OK; TCSF; Basigin(Ok Blood Group); Collagenase stimulatory factor; OK blood group antigen; Tumor cell-derived collagenase stimulatory factor
Species: Rat
Assay Type: Sandwich
Sensitivity: 5.5 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: Serum, plasma, lung lavage fluid and other biological fluids.
Assay Length: 4.5h
Research AreaResearch Area: CD & Adhesion molecule;Tumor immunity;Infection immunity;Reproductive science;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Extracellular Matrix Metalloproteinase Inducer(EMMPRIN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Extracellular Matrix Metalloproteinase Inducer(EMMPRIN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Extracellular Matrix Metalloproteinase Inducer(EMMPRIN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Extracellular Matrix Metalloproteinase Inducer(EMMPRIN) in the samples is then determined by comparing the OD of the samples to the standard curve.
