Product Description
Rat EMILIN1(Elastin Microfibril Interface Located Protein 1) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: EMI; gp115; Elastin Microfibril Interfacer 1
Species: Rat
Assay Type: Sandwich
Sensitivity: 0.124 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: tissue homogenates, cell lysates, cell culture supernates and other biological fluid
Assay Length: 4.5h
Research AreaResearch Area: Metabolic pathway;Cardiovascular biology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Elastin Microfibril Interface Located Protein 1(EMILIN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Elastin Microfibril Interface Located Protein 1(EMILIN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Elastin Microfibril Interface Located Protein 1(EMILIN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Elastin Microfibril Interface Located Protein 1(EMILIN1) in the samples is then determined by comparing the OD of the samples to the standard curve.
