Product Description
Rat COL9a1(Collagen Type IX Alpha 1) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: COL9-A1; MED; Collagen Alpha-1(IX)chain
Species: Rat
Assay Type: Sandwich
Sensitivity: 0.58 ng/mL
Standard: 100 ng/mL
Detection Range: 1.57-100 ng/mL
Sample Type: Tissue homogenates and other biological fluids.
Assay Length: 4.5h
Research AreaResearch Area: Metabolic pathway;Ophthalmology & Otorhinolaryngology;Developmental science;Nutrition metabolism;Rheumatology;Autoimmunity;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagen Type IX Alpha 1(COL9a1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Collagen Type IX Alpha 1(COL9a1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Collagen Type IX Alpha 1(COL9a1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Collagen Type IX Alpha 1(COL9a1) in the samples is then determined by comparing the OD of the samples to the standard curve.
