Product Description
Rat COL1a1(Collagen Type I Alpha 1) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: COL-1A1; COL1-A1; COL1A-1; OI4; Collagen Alpha-1(I)chain
Species: Rat
Assay Type: Sandwich
Sensitivity: 13.7 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.25-2000 pg/mL
Sample Type: serum, plasma, tissue homogenates and other biological fluids
Assay Length: 4.5h
Research AreaResearch Area: Metabolic pathway;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagen Type I Alpha 1(COL1a1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Collagen Type I Alpha 1(COL1a1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Collagen Type I Alpha 1(COL1a1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Collagen Type I Alpha 1(COL1a1) in the samples is then determined by comparing the OD of the samples to the standard curve.
