Product Description
Rat CNP(C-Type Natriuretic Peptide) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names:
Species: Rat
Assay Type: Sandwich
Sensitivity: 12.4 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.25-2000 pg/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Length: 4.5h
Research AreaResearch Area: Cardiovascular biology;Hormone metabolism;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to C-Type Natriuretic Peptide(CNP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to C-Type Natriuretic Peptide(CNP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain C-Type Natriuretic Peptide(CNP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of C-Type Natriuretic Peptide(CNP) in the samples is then determined by comparing the OD of the samples to the standard curve.
