Product Description
Rat C5(Complement Component 5) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: CPAMD4; C5a anaphylatoxin; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4
Species: Rat
Assay Type: Sandwich
Sensitivity: 0.142 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Serum, plasma and other biological fluids
Assay Length: 4.5h
Research AreaResearch Area: Metabolic pathway;Infection immunity;Immune molecule;Hematology;Autoimmunity;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Component 5(C5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Complement Component 5(C5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement Component 5(C5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Complement Component 5(C5) in the samples is then determined by comparing the OD of the samples to the standard curve.
