Product Description
Rat BLC1(B-Lymphocyte Chemoattractant 1) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: CXCL13; SCYB13; BLC; BCA1; BLR1L; ANGIE; ANGIE2; Chemokine(C-X-C-Motif)ligand 13; Small Inducible Cytokine B Subfamily(Cys-X-Cys Motif)Member 13
Species: Rat
Assay Type: Sandwich
Sensitivity: 6.5 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 4.5h
Research AreaResearch Area: Cytokine;Tumor immunity;Infection immunity;Immune molecule;Hematology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to B-Lymphocyte Chemoattractant 1(BLC1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to B-Lymphocyte Chemoattractant 1(BLC1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain B-Lymphocyte Chemoattractant 1(BLC1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of B-Lymphocyte Chemoattractant 1(BLC1) in the samples is then determined by comparing the OD of the samples to the standard curve.
