Product Description
Rat AhR(Aryl Hydrocarbon Receptor) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: BHLHE76; Class E basic helix-loop-helix protein 76
Species: Rat
Assay Type: Sandwich
Sensitivity: 0.102 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Tissue homogenates and other biological fluids.
Assay Length: 4.5h
Research AreaResearch Area: Signal transduction;Tumor immunity;Infection immunity;Dermatology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aryl Hydrocarbon Receptor(AhR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aryl Hydrocarbon Receptor(AhR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aryl Hydrocarbon Receptor(AhR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Aryl Hydrocarbon Receptor(AhR) in the samples is then determined by comparing the OD of the samples to the standard curve.
