Product Description
Rat a2M(Alpha-2-Macroglobulin) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: α2MG; α2Mg; CPAMD5; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5
Species: Rat
Assay Type: Sandwich
Sensitivity: 5.9 ng/mL
Standard: 800 ng/mL
Detection Range: 12.5-800 ng/mL
Sample Type: serum, plasma, urine and other biological fluids
Assay Length: 4.5h
Research AreaResearch Area: Tumor immunity;Infection immunity;Hematology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-2-Macroglobulin(a2M). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alpha-2-Macroglobulin(a2M). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-2-Macroglobulin(a2M), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alpha-2-Macroglobulin(a2M) in the samples is then determined by comparing the OD of the samples to the standard curve.
