Product Description
Rat a1M(Alpha-1-Microglobulin/Bikunin Precursor) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: AMBP; UTI; HCP; EDC1; HI30; IATIL; ITILC; ITI; ITIL; Alpha 1 Microglobulin/Bikunin Precursor; Growth-inhibiting protein 19; Uristatin; Uronic-Acid-Rich Protein; Trypstatin
Species: Rat
Assay Type: Sandwich
Sensitivity: 0.69 ng/mL
Standard: 100 ng/mL
Detection Range: 1.57-100 ng/mL
Sample Type: Serum, plasma, urine and other biological fluids
Assay Length: 4.5h
Research AreaResearch Area: Infection immunity;Immune molecule;Kidney biomarker;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-1-Microglobulin/Bikunin Precursor(a1M). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alpha-1-Microglobulin/Bikunin Precursor(a1M). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-1-Microglobulin/Bikunin Precursor(a1M), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alpha-1-Microglobulin/Bikunin Precursor(a1M) in the samples is then determined by comparing the OD of the samples to the standard curve.
