RASGRF1(Phospho-S916) polyclonal antibody

Product Description

RASGRF1(Phospho-S916) polyclonal antibody is available at gentaur for next week delivery

Background: A critical step in signal transduction responses to stimulation of cell surface receptors by their ligands involves the accumulation of Ras proteins in their active GTP-bound state. To reach their active GTP-bound state, Ras proteins must first release bound GDP, a rate-limiting step mediated by a guanine nucleotide releasing factor (GRF). The mammalian Ras p21 GRF protein has been designated Ras-GRF p140. Ras-GRF accelerates release of GDP from H- and N-Ras p21 protein in vitro, but not from the related Ral A or Cdc42Hs GTP-binding proteins. Of interest, a region mapping within the amino terminal domain of Ras-GRF is similar to both the human breakpoint cluster protein, Bcr, and the Dbl proto-oncogene product, a guanine nucleotide-releasing factor for CDC42Hs. Ras-GRF2 p135 has also been identified. Ras-GRF2 p135 is highly homologous to Ras-GRF1 p140 except in the region between the REM and CDC25 domains and appears to function similarly to Ras-GRF1 p140.

Applications:  WB

Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability:  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity:  RASGRF1(Phospho-S916) polyclonal antibody detects endogenous levels of RASGRF1 protein only when phosphorylated at Ser916.

W4BiowMW:  ~ 145 kDa

Reactivity: Human

Note:  For research use only, not for use in diagnostic procedure.

Immunogen:

Synthetic phosphopeptide derived from human RASGRF1 around the phosphorylation site of Serine 916.

Alternative Name:

Ras-specific guanine nucleotide-releasing factor 1; Ras-GRF1; Guanine nucleotide-releasing protein; GNRP; Ras-specific nucleotide exchange factor CDC25; RASGRF1; CDC25; GNRP; GRF1

Western blot analysis:  Western blot (WB) analysis of RASGRF1(Phospho-S916) polyclonal antibody at 1:500 dilution Lane1:U-87MG whole cell lysate(40ug) Lane2:H1792 whole cell lysate(40ug) Lane3:Panc1 whole cell lysate(40ug) Lane4:A549 whole cell lysate(40ug)

Immunohistochemistry:

Immunofluorescence analysis:

Host:  Rabbit

Swiss-Prot: Q13972

 

 

 

 

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