Product Description
Raf-B (phospho-S445) polyclonal antibody is available at gentaur for next week delivery
Background: Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. Raf-A, a second member of the Raf gene family of serine/threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain of the two molecules, but less homology elsewhere. Expression of Raf-B is highly restricted with highest levels in the cerebrum and testis.
Applications: WB
Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: P-Raf-B (S445) polyclonal antibody detects endogenous levels of Raf-B protein when phosphorylated at Ser445.
W4BiowMW: ~ 110 kDa
Reactivity: Human,Rat
Note: For research use only, not for use in diagnostic procedure.
Immunogen:
Synthetic phosphopeptide derived from human Raf-B around the phosphorylation site of Serine 445.Alternative Name:
Serine/threonine-protein kinase B-raf; Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1; BRAF; BRAF1; RAFB1Western blot analysis: Western blot analysis of extracts of Raji cells, using Phospho-BRAF-S445 antibody.
Immunohistochemistry:
Immunofluorescence analysis:
Host: Rabbit
Swiss-Prot: P15056
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