Product Description
Rabbit APOE(Apolipoprotein E) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: Apo-E; AD2; Apoprotein; Alzheimer Disease 2(E4-Associated,Late Onset
Species: Rabbit
Assay Type: Competitive Inhibition
Sensitivity: 182.5 pg/mL
Standard: 40000 pg/mL
Detection Range: 625-40000 pg/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Length: 2h
Research AreaResearch Area: Metabolic pathway;Cardiovascular biology;Hepatology;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Apolipoprotein E(APOE) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Apolipoprotein E(APOE). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Apolipoprotein E(APOE) in the samples is then determined by comparing the OD of the samples to the standard curve.