Polyclonal Antiserum

Goat anti-Alpha-Synuclein antibody

Cat. No. RDI-ASYNUCabG   $315.00/0.25ml


-A goat antiserum to a synthetic peptide that corresponds to amino acids 116 - 131 of the C-terminus of the Parkinson disease Alpha-synuclein protein is currently available. The Alpha-synuclein gene was discovered on chromosome 4 and is responsible for early-onset autosomal dominant Parkinson’s disease. It is an abundant protein whose major site of expression is in the nervous system. Human ALPHA-synuclein is also known to be a precursor of the non- -amyloid component protein (NACP) of Alzheimer’s disease where one of its fragments associates amyloid deposits.

-The function of this gene product is currently unknown, but it is being shown to be associated with other neurodegenerative diseases, such as dementia with Lewy bodies and multiple-system atrophy. This antiserum has been shown to be immunoreactive to the ALPHA-synuclein immunizing peptide by ELISA. Currently, this antibody has been shown to immunolabel Lewy bodies in Parkinson brains using formalin-fixed, paraffin-embedded sections. This antibody should be a valuable tool for scientists who will be working to discover the function of this protein in normal individuals and its role in Parkinson’s and other neurodegenerative diseases.

-References:

-Spillantini M. G., et al. A-synuclein in Lewy bodies. Nature 388: 839-840, 1997.

-Gai, W. P., et al. Multiple-system atrophy: a new A-synuclein disease. Lancet 352: 547-548, 1998.

DATA SHEET: POLYCLONAL ANTISERUM

 DESCRIPTION: ALPHA -Synuclein C-terminal Peptide Antiserum

PRODUCT NO: RDI-ASYNUCabG $312.00/0.25ml

LOT NO:

ANTIBODY HOST: Goat

IMMUNOGEN: Synthetic peptide corresponding to amino acids 116-131 of the C-terminus of the human ALPHA -Synuclein protein. 

Antigen sequence: M P V D P D N E A Y E M P S E E  (cat#RDI-ASYNUC-CPX $469.00/1mg)

FORM: Whole antiserum

CONCENTRATION: N/A

TITER: Recommended dilutions based of neat serum:
-   ELISA using immunizing peptide: approx 1:1,000
-  IHC formalin-fixed, paraffin sections: approx 1:300
-  IHC paraformaldehyde-fixed, frozen sections: n/d
-Western Blots: n/d

SPECIFICITY:Specific for residues 116-131 on the C-terminus of human ALPHA-Synuclein Protein 

STORAGE: 4° C short term; -20° long term