Product Description
POLE3 polyclonal antibody is available at gentaur for next week delivery
Background: DNA replication is initiated by the binding of initiation factors to the origin of replication. Nucleosomes inhibit access to the replication machinery at these origin sequences. Nucleosome remodeling factors increase the accessibility of nucleosomal DNA to transcriptional regulators . CHRAC15 and CHRAC17 are subunits of the nucleosomal remodeling factor CHRAC (chromatin accessibility complex),which increases the accessibility of nucleosomal DNA in an ATP-dependent manner . Unlike other known chromatin remodelling factors, CHRAC also functions during chromatin assembly by using ATP to convert irregular chromatin into a regular array of nucleosomes with even spacing . This conversion process occurs when CHRAC organizes randomly deposited histones into a regularly spaced array . In the presence of CHRAC, the nucleosomal ATPase ISWI catalyses several ATP-dependent transitions of chromatin structure .
Applications: WB IF
Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: POLE3 polyclonal antibody detects endogenous levels of POLE3 protein.
W4BiowMW: ~ 20-25 kDa
Reactivity: Human,Mouse
Note: For research use only, not for use in diagnostic procedure.
Immunogen:
Recombinant full length Human POLE3.Alternative Name:
DNA polymerase epsilon subunit 3; Arsenic-transactivated protein; AsTP; Chromatin accessibility complex 17 kDa protein; CHRAC-17; HuCHRAC17; DNA polymerase II subunit 3; DNA polymerase epsilon subunit p17; POLE3; CHRAC17Western blot analysis: Western blot analysis of POLE3 antibody.
Immunohistochemistry: Immunofluorescence analysis of U2OS cell using POLE3 antibody.
Immunofluorescence analysis: Immunofluorescence analysis of U2OS cell using POLE3 antibody. Green:gamma H2AX (phospho S139) used for DNA damage marker. Blue: DAPI for nuclear staining. Cells were treated by UV-A laser.
Host: Rabbit
Swiss-Prot: Q9NRF9
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