Product Description
PLC β3 (L532) polyclonal antibody is available at gentaur for next week delivery
Background: A total of eight mammalian PLC isozymes have been described (PLC β1, PLC β2, PLC β3, PLC β4, PLC γ1, PLC γ2, PLC δ1 and PLC δ2) with molecular weights ranging from 85 to 150 kDa. The γ-type enzymes are unique in that they contain SH2 and SH3 domains. Moreover, the two γ-type enzymes, but not the β and δ isozymes, are subject to activation by a number of protein tyrosine kinases which associate with their SH2 domains and induce their activation by phosphoryation. In contrast, activation of PLC β1, PLC β2 and PLC β3 is mediated by the α subunits of the Gq class of heterotrimeric G proteins and by certain βγ G protein subunits. The regulatory mechanisms for PLC δ1 and PLC δ2 are as yet not resolved.
Applications: WB IHC IF
Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: PLC β3 (L532) polyclonal antibody detects endogenous levels of PLC β3 protein.
W4BiowMW: ~ 150 kDa
Reactivity: Human,Mouse,Rat
Note: For research use only, not for use in diagnostic procedure.
Immunogen:
Synthetic peptide, corresponding to amino acids 500-550 of Human PLC β3.Alternative Name:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase β-3; Phosphoinositide phospholipase C-β-3; Phospholipase C-β-3; PLC-β3; 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3; Phosphoinositide phospholipase C-beta-3; Phospholipase C-beta-3; PLC-beta-3; PLCB3; PLCβ3Western blot analysis: Western blot (WB) analysis of PLCB3 pAb at 1:500 dilution Lane1:HCT116 whole cell lysate(40ug) Lane2:Beas-2B whole cell lysate(40ug) Lane3:PMVEC whole cell lysate(40ug) Lane4:CT26 whole cell lysate(40ug)
Immunohistochemistry: Immunohistochemistry (IHC) analyzes of PLC β3 (L532) pAb in paraffin-embedded human breast carcinoma tissue.
Immunofluorescence analysis:
Host: Rabbit
Swiss-Prot: Q01970
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