Product Description
PLC β2 polyclonal antibody is available at gentaur for next week delivery
Background: Phosphoinositide-specific phospholipase C (PLC) plays a crucial role in the initiation of receptor mediated signal transduction through the generation of the two second messengers, inositol 1,4,5-triphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. There are many mammalian PLC isozymes, including PLC β1, PLC β2, PLC β3, PLC β4, PLC γ1, PLC γ2, PLC δ1,PLC δ2 and PLC ε). PLC βs are the only PLC isforms that are regulated by G protein subunits and are activated by a heterotrimeric GTP-binding protein linked to various cell surface receptors. Two alternatively spliced forms (1,181 and 1,166 amino acids) of PLC β2 are generated in hematopoietic cells that differ in the carboxyl-terminal sequence implicated in interaction of PLC β enzymes with Gαq.The pleckstrin homology domain of PLC β2 is required for its targeting to the membrane and for substrate hydrolysis and its linker region exerts an inhibitory efect on basal PLC β2 activity. PLC β2 plays a major role in platelet activation and is mainly expressed in the periphery of the islet and acinar cells in rat pancreas.
Applications: WB IHC
Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: PLC β2 polyclonal antibody detects endogenous levels of PLC β2 protein.
W4BiowMW: ~ 150 kDa
Reactivity: Human,Mouse,Rat
Note: For research use only, not for use in diagnostic procedure.
Immunogen:
Recombinant protein of human PLCB2.Alternative Name:
PLC-beta-2;Western blot analysis: Western blot analysis of extract of various cells, using PLCB2 antibody.
Immunohistochemistry: Immunohistochemistry of paraffin-embedded human stomach using PLCB2 antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis:
Host: Rabbit
Swiss-Prot: Q00722
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