Product Description
PKR polyclonal antibody is available at gentaur for next week delivery
Background: An interferon-inducible, RNA-dependent protein serine/threonine kinase (PKR) has been described. PKR in earlier literature is variously known as DAI, dsJ, PI kinase, p65, p67 or TIK for the mouse kinase; and p68 or p69 for the human kinase. The PKR kinase substrate is the α subunit of protein synthesis initiation factor eIF-2. Phosphorylation of eIF-2α on serine-51 results in inhibition of translation. Molecular cDNA clones have been isolated from both human and mouse cells. The serine/threonine kinase catalytic domains map to the carboxy terminal half of the protein while the RNA-binding domains are located in the amino terminal region. Three kinds of regulation of PKR enzymatic activity have been described.
Applications: WB IHC IF
Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: PKR polyclonal antibody detects endogenous levels of PKR protein.
W4BiowMW: 74kDa
Reactivity: Human,Mouse,Rat
Note: For research use only, not for use in diagnostic procedure.
Immunogen:
Recombinant protein of human Eif2ak2Alternative Name:
Interferon-induced; double-stranded RNA-activated protein kinase; Eukaryotic translation initiation factor 2-alpha kinase 2; eIF-2A protein kinase 2; Interferon-inducible RNA-dependent protein kinase; P1/eIF-2A protein kinase; Protein kinase RNA-activated; PKR; Tyrosine-protein kinase EIF2AK2; p68 kinase; PKR; PRKR; EIF2AK2Western blot analysis: Western blot analysis of extracts of various cell lines, using Eif2ak2 antibody at 1:1000 dilution.
Immunohistochemistry: Immunohistochemistry of paraffin-embedded human liver using EIF2AK2 antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis:
Host: Rabbit
Swiss-Prot: P19525
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