Product Description
PKD1 (phospho-S205) polyclonal antibody is available at gentaur for next week delivery
Background: Activation of PKC is one of the earliest events in a cascade leading to a variety of cellular responses such as secretion, gene expression, proliferation and muscle contraction. Protein kinase D (PKD), also called PKCμ, is a serine/threonine kinase whose activation is dependent on the phosphorylation of two activation loop sites, Ser744 and Ser748, via a PKC-dependent signaling pathway. In addition to the two activation loop sites, the carboxy-terminal Ser916 has been identified as an autophosphorylation site for PKD/PKCμ. Phosphorylation at Ser916 correlates with PKD/PKCμ catalytic activity.
Applications: WB
Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: PRKD1(Phospho-S205) polyclonal antibody detects endogenous levels of PRKD1 protein only when phosphorylated at Ser205.
W4BiowMW: ~ 115 kDa
Reactivity: Human,Rat,Mouse
Note: For research use only, not for use in diagnostic procedure.
Immunogen:
Synthetic phosphopeptide derived from human PRKD1 around the phosphorylation site of Serine 205.Alternative Name:
Serine/threonine-protein kinase D1; Protein kinase C mu type; Protein kinase D; nPKC-D1; nPKC-mu; PRKD1; PKD; PKD1; PRKCMWestern blot analysis: Western blot (WB) analysis of PRKD1(Phospho-S205) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:HEK293T treated with PMA(50ng/ml,10 minutes) whole cell lysate Lane3:HEK293T treated with PMA(50ng/ml,30 minutes) whole cell lysate Lane4:H9C2 whole cell lysate Lane5:3T3-L1 whole cell lysate
Immunohistochemistry:
Immunofluorescence analysis:
Host: Rabbit
Swiss-Prot: Q15139
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