Product Description
PKC beta (Phospho-S661) polyclonal antibody is available at gentaur for next week delivery
Background: PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes including conventional (c) PKC isoforms (α, βI, βII and γ) and novel (n) PKC isoforms (δ, ε, ζ, η and θ). Patterns of expression for each PKC isoform differs among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of nPKC δ and ε are independent of Ca2+. On the other hand, nPKC δ and ε, as well as all of the cPKC members, possess phorbol ester-binding activities and kinase activities.
Applications: WB IHC
Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: PKC beta (Phospho-S661) polyclonal antibody detects endogenous levels of PKC beta protein only when phosphorylated at Ser661.
W4BiowMW: ~ 80 kDa
Reactivity: Human,Mouse,Rat
Note: For research use only, not for use in diagnostic procedure.
Immunogen:
Synthetic phosphopeptide derived from human PKC beta around the phosphorylation site of Serine 661.Alternative Name:
Protein kinase C beta type; PKC-B; PKC-beta; PKCB; PRKCB1; PRKCBWestern blot analysis: Western blot (WB) analysis of PKC beta (Phospho-S661) polyclonal antibody at 1:500 dilution LaneA:The Brain tissue lysate of Mouse
Immunohistochemistry:
Immunofluorescence analysis:
Host: Rabbit
Swiss-Prot: P05771
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