Product Description
PKC alpha polyclonal antibody is available at gentaur for next week delivery
Background: Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes including conventional (c) PKC isoforms (α, βI, βII and γ) and novel (n) PKC isoforms (δ, ε, ζ, η and θ). Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of nPKC δ and ε are independent of Ca2+.
Applications: WB IHC
Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: PKC polyclonal antibody detects endogenous levels of PKC protein.
W4BiowMW: ~ 85 kDa
Reactivity: Human,Mouse,Rat
Note: For research use only, not for use in diagnostic procedure.
Immunogen:
Synthetic peptide, corresponding to Human PKC.Alternative Name:
Protein kinase C alpha type; PKC-A; PKC-alpha; PRKCA; PKCA; PRKACAWestern blot analysis: Western blot (WB) analysis of PKC alpha polyclonal antibody at 1:500 dilution LaneA:Hela whole cell lysate LaneB:COS-7 whole cell lysate LaneC:HEK293T whole cell lysate LaneD:C6 whole cell lysate LaneE:CT26 whole cell lysate
Immunohistochemistry:
Immunofluorescence analysis:
Host: Rabbit
Swiss-Prot: P17252
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