Product Description
Pig vWF(Von Willebrand Factor) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: F8VWF; VWD; von Willebrand antigen 2
Species: Pig
Assay Type: Competitive Inhibition
Sensitivity: 4.83 ng/mL
Standard: 1000 ng/mL
Detection Range: 15.63-1000 ng/mL
Sample Type: Plasma
Assay Length: 2h
Research AreaResearch Area: Signal transduction;Hematology;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Von Willebrand Factor(vWF) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Von Willebrand Factor(vWF). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Von Willebrand Factor(vWF) in the samples is then determined by comparing the OD of the samples to the standard curve.
