Product Description
Pig FABP2(Intestinal Fatty Acid Binding Protein) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: FABPI; IFABP; I-FABP; Intestinal-type fatty acid-binding protein
Species: Pig
Assay Type: Sandwich
Sensitivity: 6.4 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 4.5h
Research AreaResearch Area: Metabolic pathway;Tumor immunity;Endocrinology;Cardiovascular biology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Intestinal Fatty Acid Binding Protein(IFABP/FABP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Intestinal Fatty Acid Binding Protein(IFABP/FABP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Intestinal Fatty Acid Binding Protein(IFABP/FABP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Intestinal Fatty Acid Binding Protein(IFABP/FABP2) in the samples is then determined by comparing the OD of the samples to the standard curve.
