Product Description
PFKFB3 polyclonal antibody is available at gentaur for next week delivery
Background: Phosphofructosekinase-2 (PFK-2) belongs to the phosphoglycerate mutase family and is required for the activation of cellular glycolysis. Within the glycolysis pathway, PFK-2 regulates the synthesis and degradation of fructose 2,6-bisphosphate (F2,6BP) by enzymatically catalyzing the phosphorylation of fructose-6-phosphate to form F2,6BP. F2,6BP functions as a potent activator for 6-phosphofructo-1-kinase that can then activate the glycolysis pathway. Various tissue-specific isoforms of PFK-2 are expressed, including the PFK-2 specific to the brain (br), the liver (liv) and the placenta (pl), and they are also differentially regulated and function as homodimers. A unique isoform, iPFK-2, is induced following proinflammatory stimuli, and it is also constituitively expressed in a variety of carcinoma cell lines, where it leads to an accumation of intracellular F2,6BP. In addition, the expression of iPFK-2 correlates to increases in DNA sysnthesis, suggesting that iPFK-2 may contribute to cellular transformation of cells and enhanced cellular proliferation.
Applications: WB IF
Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: PFKFB3 polyclonal antibody detects endogenous levels of PFKFB3 protein.
W4BiowMW: Refer to figures
Reactivity: Human,Mouse
Note: For research use only, not for use in diagnostic procedure.
Immunogen:
Recombinant full length Human PFKFB3.Alternative Name:
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3; 6PF-2-K/Fru-2,6-P2ase 3; PFK/FBPase 3; 6PF-2-K/Fru-2,6-P2ase brain/placenta-type isozyme; Renal carcinoma antigen NY-REN-56; iPFK-2Western blot analysis:
Immunohistochemistry: Immunofluorescence analysis of U2OS cell using PFKFB3 antibody. Blue: DAPI for nuclear staining.
Immunofluorescence analysis:
Host: Rabbit
Swiss-Prot: Q16875
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