Paxillin (L25) polyclonal antibody

Product Description

Paxillin (L25) polyclonal antibody is available at gentaur for next week delivery

Background: Paxillin is a multidomain protein that localizes primarily to focal adhesion sites in the extracellular matrix. Paxillin is one of the key components of integrin signaling, and tyrosine phosphorylation of paxillin is required for integrin-mediated cytoskeletal reorganization. Paxillin is phosphorylated by another focal adhesion component, focal adhesion kinase (FAK), at Tyr118. Phospho-Paxillin (Tyr118) may provide a docking site for recruitment of other signaling molecules to focal adhesions. It has been shown that the SH2 domain of Crk binds to the phosphorylated Tyr118 of paxillin.

Applications:  WB IHC IF

Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability:  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity:  Paxillin (L25) polyclonal antibody detects endogenous levels of Paxillin protein.

W4BiowMW:  ~ 54,68 kDa

Reactivity: Human,Mouse,Rat

Note:  For research use only, not for use in diagnostic procedure.

Immunogen:

Synthetic peptide, corresponding to amino acids N-terminus of Human Paxillin.

Alternative Name:

Paxillin; PXN

Western blot analysis:  Western blot (WB) analysis of PXN polyclonal antibody at 1:1000 dilution Lane1:MCF-7 whole cell lysate(40ug) Lane2:Hela whole cell lysate(40ug) Lane3:H460 whole cell lysate(40ug) Lane4:PC12 whole cell lysate(40ug) Lane5:CT-26 whole cell lysate(40ug)

Immunohistochemistry: Immunohistochemistry (IHC) analyzes of Paxillin (L25) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Immunofluorescence analysis:

Host:  Rabbit

Swiss-Prot: P49023

 

 

 

 

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