PAK4/5/6 (V469) polyclonal antibody

Product Description

PAK4/5/6 (V469) polyclonal antibody is available at gentaur for next week delivery

Background: PAK4 is highly expressed in prostate, testis and colon. PAK4 interacts tightly with GTP-bound but not GDP-bound CDC42 and weakly with RAC. PAK4 phosphorylates and autophosphorylates and also activates the JNK pathway. PAK5, a member of the PAK family of protein kinases contains a CDC42/Rac1 interactive binding (CRIB) motif at the N-terminus and a Ste20-like kinase domain at the C-terminus. PAK5 preferentially binds to CDC42 in the presence of GTP and the CRIB motif is essential for this interaction.PAK6 protein cotranslocates into the nucleus with androgen receptor, which is a steroid hormone-dependent transcription factor that is important for male sexual differentiation and development, in response to androgen.

Applications:  WB IP

Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability:  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity:  PAK4/5/6 (V469) polyclonal antibody detects endogenous levels of PAK4, PAK5 and PAK6 protein.

W4BiowMW:  ~ 64 kDa (PAK4), 80 kDa (PAK5), 75 kDa (PAK6)

Reactivity: Human,Mouse,Rat

Note:  For research use only, not for use in diagnostic procedure.

Immunogen:

Synthetic peptide, corresponding to amino acids 441-490 of Human PAK4.

Alternative Name:

Serine/threonine-protein kinase PAK 4; p21-activated kinase 4; PAK-4; PAK4; KIAA1142; Serine/threonine-protein kinase PAK 6; PAK-5; p21-activated kinase 6; PAK-6; PAK6; PAK5; Serine/threonine-protein kinase PAK 7; p21-activated kinase 5; p21-activated kinase 7; PAK-7; PAK7; KIAA1264

Western blot analysis:  Western blot (WB) analysis of PAK4/5/6 (V469) pAb at 1:500 dilution Lane1:H1792 whole cell lysate(20ug) Lane2:MCF-7 whole cell lysate(20ug) Lane3:PC3 whole cell lysate(20ug) Lane4:The Testis tissue lysate of Mouse(40ug) Lane5:The Testis tissue lysate of Rat(40ug)

Immunohistochemistry:

Immunofluorescence analysis: Immunoprecipitation of HEK293T cell lysate using PAK4/5/6 (V469) polyclonal antibody (Sepharose Bead Conjugate) #BD0048(lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate) #BD0048 (lane 4 ).Lane 1 is 30% input.The western blot was probed using PAK4/5/6 (V469) #BS3247.“↑”（supernatant）;“↓(deposition）

Host:  Rabbit

Swiss-Prot: O96013/Q9NQU5/Q9P286

 

 

 

 

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