Product Description
p47-phox (phospho-S370) polyclonal antibody is available at gentaur for next week delivery
Background: The heredity disease chronic granulomatous disease (CGF) has been linked to mutations in p47-phox and p67-phox. The cytosolic proteins p47-phox and p67-phox, also designated neutrophil cytosol factor (NCF)1 and NCF2, respectively, are required for activation of the superoxide-producing NADPH oxidase in neutrophils and other phagocytic cells. During activation of the NADPH oxidase, p47-phox and p67-phox migrate to the plasma membrane where they associate with cytochrome b558 and the small G protein Rac to form the functional enzyme complex. Both p47-phox and p67-phox contain two Src homology 3 (SH3) domains. The C-terminal SH3 doamin of p67-phox has been shown to interact with the proline rich domain of p47-phox, suggesting that p47-phox may faciliate the transport of p67-phox to the membrane.
Applications: WB IHC ICC IP
Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: p-p47-phox (S370) polyclonal antibody detects endogenous levels of p47-phox protein only when phosphorylated at Ser370.
W4BiowMW: ~ 45 kDa
Reactivity: Human,Mouse,Rat
Note: For research use only, not for use in diagnostic procedure.
Immunogen:
Synthetic phosphopeptide derived from human p47-phox around the phosphorylation site of Serine 370.Alternative Name:
Neutrophil cytosol factor 1; NCF-1; 47 kDa autosomal chronic granulomatous disease protein; 47 kDa neutrophil oxidase factor; NCF-47K; Neutrophil NADPH oxidase factor 1; Nox organizer 2; Nox-organizing protein 2; SH3 and PX domain-containing protein 1A; p47-phox; NCF1; NOXO2; SH3PXD1AWestern blot analysis: Western blot (WB) analysis of p-p47-phox (S370) polyclonal antibody at 1:500 dilution Lane1:Hela cell treated with serum starvation(24h) Lane2:Raw264.7 cell treated with serum starvation(24h) Lane3:H9C2 cell treated with serum starvation(24h)
Immunohistochemistry: Immunohistochemistry (IHC) analyzes of p-p47-phox (S370) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
Immunofluorescence analysis:
Host: Rabbit
Swiss-Prot: P14598
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