NNT polyclonal antibody

Product Description

NNT polyclonal antibody is available at gentaur for next week delivery

Background: The proccess of cellular respiration is carried out by integral inner mitochondrial membrane proteins that facilitate the harnessing of energy released by the oxidation of NADH. NNT (Nicotinamide nucleotide transhydrogenase), also known as mitochondrial NAD(P) transhydrogenase or pyridine nucleotide transhydrogenase, is a 1086 amino acid multi-pass mitochondrial inner membrane protein. NNT is a homodimer with an N-terminal section belonging to the AlaDH/PNT family and a C-terminal section belonging to the PNT β subunit family. NNT catalyzes the transfer of a hydride ion from NADH to NADP+ and functions as a mitochondrial inner membrane proton pump. Using the energy of the proton gradient created by the electron transport chain, NNT produces high concentrations of NADPH, which is used in free radical detoxification and biosynthesis.

Applications:  WB

Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability:  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity:  NNT polyclonal antibody detects endogenous levels of NNT protein.

W4BiowMW:  ~114kDa,125kDa

Reactivity: Human, Mouse, Rat

Note:  For research use only, not for use in diagnostic procedure.

Immunogen:

A synthetic peptide corresponding to residues in Human NNT.

Alternative Name:

NAD(P) transhydrogenase, mitochondrial; Nicotinamide nucleotide transhydrogenase; Pyridine nucleotide transhydrogenase

Western blot analysis:  Western blot analysis of extracts of various cell lines, using NNT antibody ,at 1:1000 dilution. Lysates/proteins: 40ug per lane. Exposure time: 5s.

Immunohistochemistry:

Immunofluorescence analysis:

Host:  Rabbit

Swiss-Prot: Q13423

 

 

 

 

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