Myc (K422) polyclonal antibody

Product Description

Myc (K422) polyclonal antibody is available at gentaur for next week delivery

Background: c-Myc-, N-Myc- and L-Myc-encoded proteins function in cell proliferation, differentiation and neoplastic disease. Myc proteins are nuclear proteins with relatively short half lives. Amplification of the c-Myc gene has been found in several types of human tumors including lung, breast and colon carcinomas, while the N-Myc gene has been found amplified in neuroblastomas. The presence of three sequence motifs in the c-Myc COOH terminus, including the leucine zipper, the helix-loop-helix and a basic region provided initial evidence for a sequence-specific binding function.

Applications:  WB IHC IF

Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability:  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity:  Myc (K422) polyclonal antibody detects endogenous levels of proto-oncogene c-Myc protein.

W4BiowMW:  ~ 55 kDa

Reactivity: Human,Mouse,Rat

Note:  For research use only, not for use in diagnostic procedure.

Immunogen:

Synthetic peptide, corresponding to amino acids 401-450 of Human Myc.

Alternative Name:

Myc proto-oncogene protein; Class E basic helix-loop-helix protein 39; bHLHe39; Proto-oncogene c-Myc; Transcription factor p64; MYC; BHLHE39

Western blot analysis:  Western blot (WB) analysis of MYC pAb at 1:1000 dilution Lane1:3T3-L1 whole cell lysate(40ug) Lane2:PC12 whole cell lysate(40ug) Lane3:A549 whole cell lysate(40ug) Lane4:Hela whole cell lysate(40ug) Lane5:HEK293T whole cell lysate(40ug)

Immunohistochemistry: Immunohistochemistry (IHC) analyzes of Myc (K422) pAb in paraffin-embedded liver cancer tissue at 1:100.

Immunofluorescence analysis:

Host:  Rabbit

Swiss-Prot: P01106

 

 

 

 

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