Product Description
Mouse TP53(Tumor Protein p53) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: p53; LFS1; TRP53; Li-Fraumeni Syndrome; Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53
Species: Mouse
Assay Type: Sandwich
Sensitivity: 2.46 pg/mL
Standard: 400 pg/mL
Detection Range: 6.25-400 pg/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Length: 4.5h
Research AreaResearch Area: Signal transduction;Metabolic pathway;Tumor immunity;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tumor Protein p53(TP53). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tumor Protein p53(TP53). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tumor Protein p53(TP53), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tumor Protein p53(TP53) in the samples is then determined by comparing the OD of the samples to the standard curve.
