Product Description
Mouse SYP(Synaptophysin) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: Major synaptic vesicle protein p38
Species: Mouse
Assay Type: Sandwich
Sensitivity: 0.264 ng/mL
Standard: 40 ng/mL
Detection Range: 0.63-40 ng/mL
Sample Type: serum, plasma, tissue homogenates, cerebrospinal fluid and other biological fluids
Assay Length: 4.5h
Research AreaResearch Area: Neuro science;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Synaptophysin(SYP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Synaptophysin(SYP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Synaptophysin(SYP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Synaptophysin(SYP) in the samples is then determined by comparing the OD of the samples to the standard curve.
