Product Description
Mouse SNCa(Synuclein Alpha) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: SNC-A; αSYN; PD1; PARK1; PARK4; NACP; Non-A Beta Component Of Alzheimer's Disease Amyloid Precursor Protein; Non-A4 component of amyloid precursor
Species: Mouse
Assay Type: Sandwich
Sensitivity: 28 pg/mL
Standard: 5000 pg/mL
Detection Range: 78.13-5000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 4.5h
Research AreaResearch Area: Signal transduction;Neuro science;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Synuclein Alpha(SNCa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Synuclein Alpha(SNCa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Synuclein Alpha(SNCa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Synuclein Alpha(SNCa) in the samples is then determined by comparing the OD of the samples to the standard curve.
