Product Description
Mouse S1PR4(Sphingosine 1 Phosphate Receptor 4) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: EDG6; LPC1; S1P4; SLP4; Endothelial Differentiation,Lysophosphatidic Acid G-Protein-Coupled Receptor 6; Sphingosine 1-phosphate receptor Edg-6
Species: Mouse
Assay Type: Sandwich
Sensitivity: 30 pg/mL
Standard: 5000 pg/mL
Detection Range: 78.13-5000 pg/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids.
Assay Length: 4.5h
Research AreaResearch Area: Signal transduction;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sphingosine 1 Phosphate Receptor 4(S1PR4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sphingosine 1 Phosphate Receptor 4(S1PR4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sphingosine 1 Phosphate Receptor 4(S1PR4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sphingosine 1 Phosphate Receptor 4(S1PR4) in the samples is then determined by comparing the OD of the samples to the standard curve.
