Product Description
Mouse S17aH(17-Alpha-Hydroxylase) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: CYP17A1; CPT7; P450C17; 17-alpha-hydroxyprogesterone aldolase/17,20-Lyase; Steroid 17-alpha-monooxygenase; Cytochrome P450,Family 17,Subfamily A,Polypeptide 1
Species: Mouse
Assay Type: Sandwich
Sensitivity: 0.58 ng/mL
Standard: 100 ng/mL
Detection Range: 1.57-100 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids
Assay Length: 4.5h
Research AreaResearch Area: Enzyme & Kinase;Metabolic pathway;Endocrinology;Cardiovascular biology;Hepatology;Gastroenterology;Nutrition metabolism;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 17-Alpha-Hydroxylase(S17aH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 17-Alpha-Hydroxylase(S17aH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 17-Alpha-Hydroxylase(S17aH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 17-Alpha-Hydroxylase(S17aH) in the samples is then determined by comparing the OD of the samples to the standard curve.
