Product Description
Mouse REG3g(Regenerating Islet Derived Protein 3 Gamma) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: REG-III; PAP1B; PAPIB; UNQ429; Pancreatitis-associated protein 1B; Regenerating islet-derived protein III-gamma
Species: Mouse
Assay Type: Sandwich
Sensitivity: 11.7 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.25-2000 pg/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 4.5h
Research AreaResearch Area: Infection immunity;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Regenerating Islet Derived Protein 3 Gamma(REG3g). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Regenerating Islet Derived Protein 3 Gamma(REG3g). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Regenerating Islet Derived Protein 3 Gamma(REG3g), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Regenerating Islet Derived Protein 3 Gamma(REG3g) in the samples is then determined by comparing the OD of the samples to the standard curve.
