Product Description
Mouse PLA2G2D(Phospholipase A2, Group IID) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: PLA2G2D; sPLA2S; s-PLA2; sPLA2; SPLASH; Secreted Phospholipase A2; Phosphatidylcholine 2-acylhydrolase 2D; Secretory-type PLA, stroma-associated homolog
Species: Mouse
Assay Type: Sandwich
Sensitivity: 13.8 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.25-2000 pg/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids
Assay Length: 4.5h
Research AreaResearch Area: Enzyme & Kinase;Metabolic pathway;Hepatology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phospholipase A2, Group IID(PLA2G2D). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phospholipase A2, Group IID(PLA2G2D). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phospholipase A2, Group IID(PLA2G2D), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phospholipase A2, Group IID(PLA2G2D) in the samples is then determined by comparing the OD of the samples to the standard curve.
