Product Description
Mouse PDPK1(Phosphoinositide Dependent Protein Kinase 1) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: PDK1; PkB-Like 1
Species: Mouse
Assay Type: Sandwich
Sensitivity: 0.055 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids.
Assay Length: 4.5h
Research AreaResearch Area: Signal transduction;Enzyme & Kinase;Endocrinology;Hormone metabolism;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphoinositide Dependent Protein Kinase 1(PDPK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phosphoinositide Dependent Protein Kinase 1(PDPK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phosphoinositide Dependent Protein Kinase 1(PDPK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phosphoinositide Dependent Protein Kinase 1(PDPK1) in the samples is then determined by comparing the OD of the samples to the standard curve.
