Product Description
Mouse MBP(Myelin Basic Protein) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: PRG2; BMPG; Proteoglycan 2; Bone Marrow,Natural Killer Cell Activator; Eosinophil Granule Major Basic Protein; Proteoglycan 2,Bone Marrow; Pregnancy-associated major basic
Species: Mouse
Assay Type: Sandwich
Sensitivity: 0.114 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates and other biological fluids
Assay Length: 4.5h
Research AreaResearch Area: Infection immunity;Rheumatology;Dermatology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myelin Basic Protein(MBP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myelin Basic Protein(MBP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myelin Basic Protein(MBP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Myelin Basic Protein(MBP) in the samples is then determined by comparing the OD of the samples to the standard curve.
