Product Description
Mouse JAG1(Jagged 1 Protein) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: CD339; AGS; AHD; AWS; HJ1; JAGL1; Alagille Syndrome
Species: Mouse
Assay Type: Sandwich
Sensitivity: 6 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: Tissue homogenates and other biological fluids
Assay Length: 4.5h
Research AreaResearch Area: Signal transduction;CD & Adhesion molecule;Tumor immunity;Hematology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Jagged 1 Protein(JAG1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Jagged 1 Protein(JAG1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Jagged 1 Protein(JAG1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Jagged 1 Protein(JAG1) in the samples is then determined by comparing the OD of the samples to the standard curve.
