Product Description
Mouse IgG1(Immunoglobulin G1) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: IGHG1; Ig Gamma-1 Chain C Region; Immunoglobulin Heavy Constant Gamma 1; G1m Marker; Immunoglobulin Gm1
Species: Mouse
Assay Type: Competitive Inhibition
Sensitivity: 259.3 ng/mL
Standard: 50000 ng/mL
Detection Range: 781.25-50000 ng/mL
Sample Type: Serum, plasma and other biological fluids
Assay Length: 2h
Research AreaResearch Area: Immune molecule;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Immunoglobulin G1(IgG1) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Immunoglobulin G1(IgG1). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Immunoglobulin G1(IgG1) in the samples is then determined by comparing the OD of the samples to the standard curve.
