Product Description
Mouse IGF1(Insulin Like Growth Factor 1) ELISA Kit is availabe at Gentaur for next week delivery.
Alternative Names: IGFI; IGF1A; IBP1; MGF; Somatomedin C; Mechano Growth Gactor
Species: Mouse
Assay Type: Sandwich
Sensitivity: 0.065 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 4.5h
Research AreaResearch Area: Tumor immunity;Endocrinology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Insulin Like Growth Factor 1(IGF1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Insulin Like Growth Factor 1(IGF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Insulin Like Growth Factor 1(IGF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Insulin Like Growth Factor 1(IGF1) in the samples is then determined by comparing the OD of the samples to the standard curve.
